MicroDiagnostics

Novorem can detect and track the abundance of any microbe of interest in groundwater samples. We use gene quantification to quantify the abundance of specific DNA sequences including phylogenetic markers (eg. the 16S rRNA gene) to track specific microbial lineages or functional genes of interest (eg. vinyl chloride reductase) per unit volume of groundwater.

We routinely quantify total numbers of bacteria and archaea and chlorinated solvent respiring genera Dehalococcoides, Dehalobacter, Dehalogenimonas and Desulfitobacterium. We also routinely quantify functional genes involved in chlorinated ethene reduction (tceA, bvcA and vcrA). Other groups of microbes targeted include methanogenic archaea, sulphate reducing bacteria and iron reducing bacteria. But if you’re interested in other organisms we can establish and validate additional quantitative PCR assays rapidly and start turning samples around without delay.

If you prefer to ask an open question, Novorem can provide a detailed description of a microbial community present in any given environment using cutting edge high throughput DNA sequencing technologies. These analyses generate thousands of DNA sequences that Novorem can summarise in a simple table describing the percentage abundance and metabolic characteristics of each of the major constituent populations of complex microbial communities. If a global overview of the biogeochemistry of your system is essential to your goal then this is the approach for you.