Gene quantification

Gene quantification is a well established technology for quantifying the abundance of a specific DNA sequence. Traditional PCR (polymerase chain reaction) simply generates a pool of copies of a DNA sequence, however the size of the pool does not reflect the quantity of the starting material. To achieve this, the amplification of the DNA sequence needs to monitored as the reaction progresses (ie. in real time) to enable quantification.

At Novorem we use a Biorad Real Time technology platform to monitor the amplification of DNA sequences in PCRs enabling accurate quantification of specific sequences in environmental samples. Results are reported as gene copies per unit volume (L) or mass (g) of groundwater or sediment samples respectively. This is a mainstay of Novorem’s services. High quality diagnostics for bioremediation practitioners.